LOCUS       CHSY1-pGEn2             7629 bp    DNA     circular     20-SEP-2016
FEATURES             Location/Qualifiers
     CDS             129..1108
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_recomb     complement(3839..3867)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6257..6798
                     /note="WPRE"
     misc_recomb     6219..6241
                     /label=attB2
                     /note="attB2 site"
                     /note="recognized as attB2"
     misc_feature    complement(6805..7019)
                     /note="BGH pA"
     misc_feature    complement(7107..7236)
                     /note="3'-ITR"
     misc_feature    2982..3056
                     /note="TCM signal sequence"
     misc_feature    3060..3083
                     /note="His tag"
     misc_feature    3084..3134
                     /note="AviTag"
     misc_feature    3135..3830
                     /note="Superfolder GFP"
     misc_feature    2976..2981
                     /note="Kozak seq"
     misc_feature    3868..3890
                     /note="TEV site"
     misc_feature    3891..6212
                     /note="OPTIMIZED CHSY1 coding region"
     vector          join(6227..7629,1..3857)
                     /source="pGEn2-DEST"
                     /type="Destination Vector"
     clone           3858..6226
                     /source="CHSY1 pDONR"
                     /type="Entry Clone"
     source          1..7629
                     /dnas_title="CHSY1-pGEn2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAgcg gccgcGCCAC cATGAGACTG CTGACCGCCC
     3001 TGTTCGCCTA CTTCATCGTG GCCCTGATCC TGGCCTTCAG CGTGTCCGCC AAGAGCATGC
     3061 ACCACCACCA TCACCATCAT CACATGAGCG GCCTGAACGA CATCTTCGAG GCCCAGAAAA
     3121 TCGAGTGGCA CGAGATGAGC AAGGGCGAGG AACTGTTCAC CGGCGTGGTG CCCATCCTGG
     3181 TGGAACTGGA CGGCGACGTG AACGGCCACA AGTTCTCTGT GCGGGGCGAG GGCGAGGGGG
     3241 ACGCCACAAA TGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGAAAG CTGCCCGTGC
     3301 CCTGGCCTAC CCTGGTCACA ACCCTGACCT ACGGCGTGCA GTGCTTCAGC AGATACCCCG
     3361 ACCACATGAA GCGGCACGAT TTCTTCAAGA GCGCCATGCC CGAGGGCTAC GTGCAAGAAC
     3421 GGACCATCAG CTTCAAGGAC GACGGCACCT ACAAGACCAG AGCCGAAGTG AAGTTCGAGG
     3481 GCGACACCCT GGTCAACCGG ATCGAGCTGA AGGGCATCGA CTTCAAAGAG GACGGCAACA
     3541 TCCTGGGCCA CAAGCTCGAG TACAACTTCA ACAGCCACAA CGTGTACATC ACCGCCGACA
     3601 AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGGCA CAACGTGGAA GATGGCAGCG
     3661 TGCAGCTGGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
     3721 CCGACAATCA CTACCTGAGC ACCCAGAGCG TGCTGAGCAA GGACCCCAAC GAGAAGCGGG
     3781 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACCCACGGC GAATTCgcAT
     3841 CAACAAGTTT GTACAAAAAA GCAGGCTctg aaaacttgta ctttcaaggc CCCAGAGCCA
     3901 GCGAGCTGAA GAGAGCTGGA CCTAGAAGAA GGGCCAGCCC AGAGGGATGT AGATCTGGAC
     3961 AGGCTGCTGC TTCTCAGGCT GGCGGAGCTA GAGGGGATGC TAGAGGCGCT CAGCTGTGGC
     4021 CTCCTGGATC TGATCCTGAT GGCGGCCCTA GAGACAGAAA CTTCCTGTTT GTGGGCGTGA
     4081 TGACCGCCCA GAAGTACCTG CAGACAAGAG CCGTGGCCGC CTACAGAACC TGGTCTAAGA
     4141 CAATCCCCGG CAAGGTGCAG TTCTTCAGCA GCGAGGGAAG CGATACCAGC GTGCCAATTC
     4201 CTGTGGTGCC ACTGAGAGGC GTGGACGATA GCTACCCCCC CCAGAAAAAG TCCTTCATGA
     4261 TGCTGAAGTA TATGCACGAC CACTACCTGG ATAAGTACGA GTGGTTCATG CGGGCCGACG
     4321 ACGACGTGTA CATCAAGGGC GACCGGCTGG AAAATTTCCT GCGGAGCCTG AATAGCTCCG
     4381 AGCCTCTGTT TCTGGGCCAG ACAGGCCTGG GCACAACAGA GGAAATGGGC AAACTGGCCC
     4441 TGGAACCCGG CGAGAATTTC TGTATGGGAG GCCCTGGCGT GATCATGAGC CGGGAAGTGC
     4501 TGAGAAGAAT GGTGCCCCAC ATCGGCAAGT GCCTGCGGGA AATGTACACC ACCCACGAGG
     4561 ATGTGGAAGT GGGCAGATGT GTGCGGAGAT TTGCTGGCGT GCAGTGCGTG TGGTCTTACG
     4621 AAATGCAGCA GCTGTTCTAC GAGAACTACG AGCAGAACAA GAAAGGCTAC ATCCGGGACC
     4681 TGCACAACTC CAAGATCCAC CAGGCCATCA CCCTGCACCC CAACAAGAAT CCCCCCTACC
     4741 AGTACCGGCT GCACAGCTAC ATGCTGAGCA GAAAGATCTC CGAGCTGCGG CACAGAACAA
     4801 TCCAGCTGCA CCGGGAAATC GTGCTGATGT CCAAGTACAG CAACACCGAG ATCCACAAAG
     4861 AGGACCTCCA GCTGGGCATC CCTCCCAGCT TCATGAGATT CCAGCCTCGG CAGCGGGAAG
     4921 AGATCCTGGA ATGGGAGTTT CTGACCGGCA AGTATCTGTA CAGCGCCGTG GATGGACAGC
     4981 CCCCTAGAAG GGGAATGGAT TCTGCCCAGA GAGAAGCCCT GGACGACATC GTGATGCAGG
     5041 TCATGGAAAT GATTAACGCC AACGCCAAGA CCCGGGGCAG GATCATCGAC TTCAAAGAGA
     5101 TTCAGTACGG CTATCGGAGA GTGAACCCTA TGTACGGCGC CGAGTACATT CTGGACCTGC
     5161 TGCTCCTCTA CAAGAAGCAC AAGGGCAAGA AAATGACCGT GCCAGTGCGC AGACACGCCT
     5221 ATCTGCAGCA GACCTTCTCT AAGATCCAGT TCGTGGAACA CGAGGAACTG GACGCCCAGG
     5281 AACTCGCCAA GAGAATCAAT CAGGAAAGCG GCTCCCTGAG CTTCCTGAGC AACAGCCTGA
     5341 AGAAACTGGT GCCCTTTCAG CTGCCCGGCT CCAAGAGCGA GCACAAAGAA CCCAAGGACA
     5401 AGAAGATCAA CATCCTGATC CCCCTGAGCG GCAGATTCGA CATGTTCGTG CGGTTCATGG
     5461 GCAACTTCGA GAAAACCTGC CTGATTCCCA ACCAGAACGT CAAGCTGGTG GTGCTGCTGT
     5521 TCAACTCCGA CAGCAACCCT GACAAGGCCA AACAGGTGGA ACTGATGCGG GACTACAGAA
     5581 TCAAGTACCC CAAGGCCGAC ATGCAGATCC TCCCTGTGTC CGGCGAATTT TCTAGAGCCC
     5641 TGGCCCTCGA AGTGGGAAGC AGCCAGTTTA ACAACGAGTC CCTGCTGTTT TTCTGCGACG
     5701 TGGACCTGGT GTTTACAACC GAATTCCTGC AGCGGTGCAG AGCCAATACT GTGCTGGGAC
     5761 AGCAGATCTA TTTCCCCATC ATCTTCAGCC AGTACGACCC CAAGATCGTG TACTCCGGAA
     5821 AGGTGCCCAG CGACAACCAC TTCGCCTTCA CACAGAAAAC CGGCTTTTGG CGGAACTACG
     5881 GCTTCGGCAT CACCTGTATC TACAAGGGGG ACCTCGTCAG AGTGGGCGGC TTCGATGTGT
     5941 CAATTCAGGG CTGGGGACTG GAAGATGTGG ACCTCTTCAA CAAGGTGGTG CAGGCCGGAC
     6001 TGAAAACCTT TAGAAGCCAG GAAGTCGGCG TGGTGCATGT GCACCATCCT GTGTTCTGCG
     6061 ATCCCAACCT GGACCCTAAG CAGTACAAGA TGTGCCTGGG CTCTAAGGCC AGCACCTACG
     6121 GATCTACACA GCAGCTCGCC GAGATGTGGC TCGAGAAGAA CGACCCTAGC TACAGCAAGA
     6181 GCAGCAACAA CAATGGCAGC GTGCGGACAG CTTAGGACCC AGCTTTCTTG TACAAAGTGG
     6241 TTGATAGCTT GGATCCAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT
     6301 TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA
     6361 TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC
     6421 TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC
     6481 TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT
     6541 CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG
     6601 GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC
     6661 CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA
     6721 CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG
     6781 GCCTCTTCCG CGTCTTCGAG ATCTGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT
     6841 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC
     6901 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
     6961 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAC
     7021 TCGAGTTAAG GGCGAATTGC CGATTAGGAT CTTCCTAGAG CATGGCTACG TAGATAAGTA
     7081 GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC
     7141 TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT
     7201 TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAAGAGC TCATGGCGCG
     7261 CCTAGGCCTT GACGGCCTTC CGCCAATTCG CCCTATAGTG AGTCGTATTA CGTCGCGCTC
     7321 ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG
     7381 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGAAAC
     7441 GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGGGA GCGCCCTGTA GCGGCCACTC
     7501 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG
     7561 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT
     7621 ACAATTTAG
//

